春季南海南部上混合层数值模拟与数值实验
科技部国家重点科技项目(97-926-05-02)和科技部攀登A项目“南海季风试验研究”资助
收稿日期：2000-11-10；修改日期：2000-12-17
刘秦玉，女，1946年3月出生，教授，博导。(E-mail:liuqy@ ougd. edu. cn)
刘秦玉 孙即霖 贾旭晶
（青岛海洋大学物理海洋教育部重点实验室，青岛，266003）
摘 要 采用一维湍动能模式对南海南部的SST及混合层进行数值模拟和数值试验。结果表明：TKE模式能够模拟南海南部的海表面温度SST以及除南海南部5月中旬以外的上混合层深度随时间变化基本特征。在5～6月，SST的日振荡主要依赖于短波辐射的日变化，风的混合作用抑制了SST的日周期振荡。春季夏季风爆发期间，南海海面潜热通量和感热通量与短
波辐射和风应力相比较，是一个对SST和混合层影响较小的量。在春季南海南部，短波辐射作用能使SST升高的最大值约为4℃；潜热和感热通量能使SST的下降的最大值为3℃ 。风应力对南海混合层深度随时间变化趋势起着决定的作用，并能使其深度加深20～30 m，而短波辐射则使混合层的深度变浅2～3 m，潜热和感热通量会使混合层的深度加深1～2 m。在春季
南海南部，热通量对混合层深度的影响与风应力相比要小得多。关键词 夏季风爆发；南海；混合层；湍动能模式；数值模拟
中图法分类号 P732 文章编号 1001-1862(2001)02-149-09
Numerical Simulation and Experiments of the Upper Mixed Layer in the South of the South China Sea in Spring
Liu Qinyu Sun Jilin Jia Xujin
(Physical Oceanography Lab., Ocean University of Qingdao, Qingdao, 266003)
Abstract In this paper, the one dimensional turbulence kinetic (TKE) model is used to simulate and do some experiments on the SST and the upper mixed layer in the south of the South China Sea (SCS). The results show that the TKE model can simulate the main features of the time variation of the SST and mixed layer depth except in the middle period of May. In May and June, the diurnal oscillation of the SST is mainly determined by the diurnal variation of solar short-wave radiation, and the mixing effect of the wind restrains the diurnal oscillation of
the SST in the south of the SCS. Compared with solar short-wave radiation and the wind stress, the latent heat and sensible heat fluxes are a minor factor in controlling the variation of the SST and the mixed layer during summer monsoon onset in the SCS. Solar short wave radiation is the main heat source to maintain the SST. In spring, the effect of short-wave radiation could make the SST in the south of the SCS increase 4℃ or less; and latent heat and sensible heat fluxes could make the SST decrease 3℃. The wind controls the depth of the upper mixed layer, and its effect could make the mixed layer grow 20～30 m, the short-wave radiation could make the mixed layer shallow 2～3 m and the latent heat and sensible heat fluxes make the mixed layer become 1～2 m deeper. In the south of the SCS the effect of heat fluxes on the mixed layer depth is smaller than the effect of wind in spring.
Key words summer monsoon onset; the South China Sea; mixed layer; TKE model; numerical simulation
1998年南海夏季风环流与动能收支的多尺度特征
教育部博士点专项基金（1999042308），海洋863青年基金（818-Q-05）和中科院南海所
环境动力实验室联合资助
收稿日期：2000-06-01;修订日期：2000-10-20
高荣珍，女，1974年9月出生，在读博士生。
高荣珍 周发 管长龙 王东晓
（1 青岛海洋大学海洋气象系，青岛，266003）
（2 青岛海洋大学物理海洋研究所，青岛，266003）
（3 中国科学院南海海洋研究所，广州，510301）
摘 要 利用1998年NCEP/NCAR日平均资料研究南海夏季风环流及动能收支的多尺度变化。结果表明：1998年南海夏季风环流在对流层高层以季节变化为主，在低空以季节内变化为主；但在整个对流层，动能收支各项的变化均表现为短周期变化过程较强，而季节变化则较弱。在夏季风爆发前后，动能收支主要取决于120d和30～60d变化，爆发阶段突出了准两周与天气尺度变化的重要作用。
关键词 南海夏季风环流;动能收支;多时间尺度
中图法分类号 P732.7 文章编号 1001-1862(2001)02-158-07
Multi-Time Scale Characteristics of Circulation and Kinetic Energy Budget During the South China Sea Summer Monsoon in 1998
Gao Rongzhen Zhou Faxiu Guan Changlong
(Department of Marine Meteorology, Ocean University of Qingdao, Qingdao, 266003)
(1 Institute of Oceanography, Ocean University of Qingdao, Qingdao, 266003)
Wang Dongxiao
(South China Sea Institute of Oceanology, Academia Sinica, Guangzhou, 510301)
Abstract Using NCEP/NCAR reanalysis daily data, the features of
multi-time scale variations of circulation and kinetic energy budget during the
South China Sea (SCS) summer monsoon in 1998 are studied. The results are as
follows: (1)The variations on 120d time scales are dominant in the high-level
circulation anomalies, and the variations on 30-60d and quasi-two weeks (QTW)
time scales are dominant in the low-level circulation anomalies. However, it is
shown that the short period process variance is stronger than the long one
(120d) as far as the terms of kinetic energy budget are concerned. (2) Before
the summer monsoon onset, the energy variation appears mainly above 300hPa, and
the kineticꗬÁ‹Љ勰¿ကЀ
橢橢쿽쿽ࠄ㐼ꖟꖟ阔lÀÀÀÀÀÀÀnj⡼⡼⡼⡼ ⢘„nj秒IJ⤨⤨⤨⤨⤨⨉⨉⨉瘭[1]瘯瘯瘯瘯瘯瘯$笄Ƞ紤X癓̹À⨉⨉⨉⨉⨉癓 and its variances
appear mainly below 300hPa. (3) The QTW oscillation and SYN variance are
significant to the SCS summer monsoon onset.Key words SCS summer monsoon circulation；kinetic energy budget；multi-time
scale variation
黄、渤海表层海温对台风过程响应数值试验
国家863-818青年基金课题(818-Q-12)资助
收稿日期：2000-05-16；修订日期：2000-07-21
苏 洁，女，1966年2月出生，博士生。
苏 洁 李 磊 鲍献文 高郭平
(青岛海洋大学海洋环境学院，青岛，266003)
摘 要 根据黄、渤海夏季由台风引起的异常海温的主要特点，构造一个简单而典型的台风过程模型，利用“近海异常海温数值预报模式”对海表层温度进行了数值试验，对台风过程中引起异常海温的各因子进行了定量分析，给出台风中心及其附近各点由各因子引起的变温率变化。试验表明，在台风作用下，冷水抽吸是引起异常低温的主要原因，大风夹卷的贡献占第二位，蒸发潜热的作用也不容忽视。数值模拟还清晰地显示出台风所引起的左弱右强的不对称降温效应以及表层暖水向外输运并在台风边缘下沉的现象。另外，还对近海异常海温预报模式的几个关键性问题及模式需改进之处进行了讨论。
关键词 黄、渤海；表层海温；台风过程；冷水抽吸；风夹卷
中图法分类号 P722.5； P722.4； P731.11 文章编号 1001-1862(2001)02-165-08
Numerical Experiment of SST Response to Typhoon Process in Yellow Sea and Bohai Sea
Su Jie Li Lei Bao Xianwen Gao Guoping
(College of Physical and Environmental Oceanography,
Ocean University of Qingdao, Qingdao, 266003)
Abstract A simple and typical typhoon process was constructed according to the primary feature of the SSTA caused by Typhoon in the Yellow Sea and the Bohai Sea. In this paper, an off-shore SSTA numerical forecasting model was employed to simulate the SST during a typhoon process in this area to further quantitatively analyze the cause of SSTA in summer. The SST changes caused by different possible factors in typhoon center and its adjacent points were given as well. The test showed that in the region disturbed by typhoon, cool water pumping was the main cause of SST's great decrease, wind entrainment played a second role; the effect of vaporing latent heat could not be ignored. Asymmetry temperature decease effect (stronger to the right) and the phenomena of surface warm water's outward transportation and downwelling at the edge caused by typhoon was clearly shown in the test. In addition, some key problems about the model are disscussed and some aspects in which the model needs to be improved are mentioned in the third section.
Key words Yellow Sea and Bohai Sea; SST; typhoon process; cold water pumping; wind entrainment
二（甲基环戊二烯）钐氯化物合成和晶体结构研究
收稿日期：2000-01-21；修订日期：2000-09-30
江 涛，女，1964年7月出生，博士，教授。
江 涛 沈 琪
(青岛海洋大学海洋药物与食品研究所,青岛,266003)
(苏州大学化学化工学院,苏州,215006)
摘 要 作者以SmCl3与(CH3Cp)Na反应分离得到了三价稀土有机配合物［（CH3Cp）2SmCl·THF］2的单晶，X-射线四圆衍射测定，该配合物属正交晶系，Pbna空间群，晶胞参数a=9.662(2),b=16.476(9),c=20.582(9)Å;α=β=γ=90°,Z=4。并分析比较［（CH3Cp）2SmCl·THF］2与其他三价稀土一氯化物的结构特点。
关键词 Sm有机金属络合物;晶体结构;合成
中图法分类号 O621.3+4 文章编号 1001-1862(2001)02-173-06
Study on the Synthesis and Molecular Structure of (CH3Cp)2SmCl·THF
Jiang Tao1 Shen Qi2
(1 Marine Drug and Food Institute, Ocean University of Qingdao, Qingdao, 266003)
(2 College of Chemistry and Chemical Engineering,Suzhou University,Suzhou,215006
Abstract The complex, (CH3Cp)2SmCl·THF, has been synthesized by the reaction of SmCl3 with two equivalents of CH3CpNa. (CH3Cp)2SmCl·THF crystallized from THF in the rhombic space group Pbna with cell constants a=9.662(2), b=16.476(9), c=20.582(9) Å; α=β=γ=90°, V=3276.5 Å ,and Do=1.69g/cm3 for Z=4. Least squares refinement of 2450 unique observed reflections led to a final R of 0.0518. We also compared the structural character of the complex with other trivalent organolanthanide chloride complexes.
Key words organo-samarium complex; molecular structure; synthesis
Effects of D-Polymannuronic Sulfate on Serum Nitric OxideLevels and Plasma Endothelin-1 and Angiotensin Ⅱ
Contents in Renovascular Hypertensive Rats (Series Ⅱ)
Supported by National “95” key project (96-C02-01)
Received: 1999-09-13; Revised: 2001-02-26
Zhu Haibo (朱海波) Geng Meiyu（耿美玉) Guan Huashi（管华诗）
(Department of Marine Pharmacology, Institute of Marine Drugs and FoodsOcean University of Qingdao, Qingdao, 266003)
Abstract The effects of D-polymannuronic sulfate (DPS) on the serum nitric oxide levels and plasma Endothelin-1 and Angiotensin II contents were studied in renovascular hypertensive rats ［twokidney one clip, Goldblatt (2K 1C)］. In the prophylactic experiment in which the effects of DPS given orally at doses of 12.50, 25.00 and 50.00 mg/kg once daily for five weeks simultaneously with the initiation of the establishment of renovascular hypertensive model were evaluated. Serum nitric oxide (NO) was determined with nitric oxide kit while plasma
Angiotensin II (Ang II) and Endothelin-1 (ET-1) were measured by radioimmunoassay. Captopril (14 mg/kg), an Angiotensin II inhibitor, was served as a positive agent. The results showed that DPS could produce a dose-dependent effect on increased serum nitric oxide levels and decreased Endothelin-1 contents, whereas the inhibitory effect of DPS on plasma Angiotensin II contents was not found in a dose-dependent manner. It implicates that the mechanisms underlying the antihypertensive effects of DPS might be associated with its actions on increasing the synthesis or release of NO and decreasing the production of Ang II and ET-1 in vivo.
Key words marine D-polymannuronic sulfate (DPS); antihypertensive effect; angiotensin II; endothelin-1; renovascular hypertensive rats
海洋硫酸多糖DPS对肾血管性高血压大鼠血清NO和血浆Ang II及ET-1含量的影响
国家“九五”科技攻关项目(96-C02-01)资助
收稿日期：1999-09-13；修订日期：2000-04-18
朱海波，男，1966年9月出生，博士，讲师。现在烟台绿叶制药有限公司工作(邮编：264003)。
朱海波 耿美玉 管华诗
（青岛海洋大学海洋药物与食品研究所，青岛，266003）
摘
要 采用肾血管性高血压大鼠模型（两肾一夹型）观察海洋硫酸多糖DPS对肾血管性高血压大鼠血清中一氧化氮(NO)和血浆中血管紧张素II（Ang
II）及内皮素-1(ET-1)含量的影响。DPS在肾血管性高血压大鼠造模第二天起分别以12.50, 25.00，50.00 mg/kg口服预防给药五周，每日给药一次。于给药前、给药后第三周和第六周分别测定动脉血压和心率。实验结束前，从每只大鼠取血6
mL，用试剂盒测定血清中NO的含量；用放射免疫法测定血浆中Ang II和ET-1的含量。血管紧张素转化酶抑制剂卡托普利（14 mg/kg）作为本实验阳性对照药。结果：DPS口服预防ꗬÁ‹Љ勰¿ကЀ
橢橢쿽쿽ࠄ㐼ꖟꖟ阔lÀÀÀÀÀÀÀnj⡼⡼⡼⡼ ⢘„nj秒IJ⤨⤨⤨⤨⤨⨉⨉⨉瘭[1]瘯瘯瘯瘯瘯瘯$笄Ƞ紤X癓̹À⨉⨉⨉⨉⨉癓ndrial DNA Cytochrome
b Gene of Chinese sea bass, Lateolabrax sp.
Supported by the National Natural Science Foundation of China (No. 39970578) and the Ministry of Education Foundation for University Key Teachers
Received: 2000-03-28; Revised: 2000-12-18
Gao Tianxiang(高天翔) Zhang Xiumei(张秀梅) Chen Dagang(陈大刚)
Zhang Meizhao(张美昭) Ren Yiping(任一平) Zhang Yaping(张亚平)
(1 Fishery College, Ocean University of Qingdao, Qingdao, 266003)
(2 Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology,the Chinese Academy of Science, Kunming, 650223)
Abstract The primers of mitochondrial DNA cytochrome b gene of Chinese sea bass were designed by referencing the sequences of Anguilla species. We successfully amplified and sequenced the 410 base pairs that encode the cytochrome b gene of mitochondrial DNA. The A, T, G and C contents of the sequence were 101bp (24.63%), 112bp (27.32%), 72bp (17.56%) and 125bp (30.49%) respectively, similar to the sequences of other species.
Key words Chinese sea bass, Lateolabrax sp.; mitochondrial DNA; cytochrome b gene; DNA sequence
中国花鲈细胞色素b基因片段的序列研究
国家自然科学基金课题(39970578)及教育部高校骨干教师基金资助
收稿日期：2000-03-28；修订日期：2000-12-18
高天翔，男，1962年6月出生，博士，教授。
高天翔 张秀梅 陈大刚 张美昭 任一平 张亚平
(1 青岛海洋大学水产学院，青岛，266003)
(2 中国科学院昆明动物研究所细胞与分子进化开放研究实验室，昆明，650223)
摘 要 参考鳗鲡等鱼类线粒体DNA序列进行了中国花鲈线粒体DNA细胞色素b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为410bp，其A、T、G、C含量分别为101bp (24.63%)、112bp (27.32%)、72bp (17.56%)、125bp (30.49%)，与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。
关键词 中国花鲈；线粒体DNA；细胞色素b基因；DNA序列
中图法分类号 Q224；S965.211；Q344+.13 文章编号 1001-1862(2001)02-185-05
一种纤毛虫的分类及形态研究——寄生于牙鲆体表溃烂组织中的指状拟舟虫
教育部重点项目（99184）、“长江学者奖励计划”项目资助
收稿日期：1999-12-17；修订日期：2000-09-24
周 丽，女，1963年9月出生，博士，副教授。
周 丽 徐奎栋 战文斌 宋微波
（国家教委水产养殖开放实验室，青岛海洋大学，青岛，266003）
摘 要 报道从牙鲆（Paralichthys olivaceus）体表溃烂组织分离的一种纤毛虫，通过活体观察、扫描电镜、蛋白银染色法、银浸法，对其形态学及纤毛图式做了研究。经鉴定，该纤毛虫为指状拟舟虫（Paralembus digitiformis Kahl，1933），隶属于寡膜纲、盾纤目、嗜污科、拟舟虫属。这是指状拟舟虫在牙鲆上兼性寄生的首次报道。
关键词 牙鲆；盾纤目；纤毛虫；指状拟舟虫；形态学
中图法分类号 S943；S941.51+4 文章编号 1001-1862(2001)02-190-05
Morphological Studies of a Ciliate from the External Ulcerative Japanese Flounder, Paralichthys Olivaceus
Zhou Li Xu Kuidong Zhan Wenbin Song Weibo
(Aquaculture Research Laboratory, Ocean University of Qingdao,Qingdao, 266003)
Abstract An external ulcerative disease frequently occurred in Japanese flounder, Paralichthys olivaceus, cultured in artificial propagation facilities along the coasts of Shandong, China, has caused mass mortalities in recent years. The ciliate was a pathogen to flounder and was identified as Paralembus digitiformis Kahl 1933, a marine facultative parasitic scuticociliatid ciliate. Morphological studies were made on living observations and specimens treated with protargolimpregnation techniques and Chatton-Lwoff silver impregnation techniques as well as scanning electron microscopy. The ciliate, Paralembus digitiformis Kahl 1933, as an opportunistic pathogen of cultured flounder, is first reported in this paper.
Key words Paralichthys olivaceus; Scuticociliatida Small; Scuticociliatida ciliate; Paralembus digitiformis; morphology
花鲈亲鱼人工培育与催产技术研究
国家自然科学基金课题（3990578）资助
收稿日期：2000-04-04；修订日期：2000-06-19
张美昭，男，1963年11月出生，工程师。
张美昭 高天翔 阮树会 庄虔增 曲永琪
(1 青岛海洋大学水产学院，青岛，266003 2 威海市羊亭水产总公司，威海，264200 3 山东省海水养殖研究所,青岛，266002)
摘 要 作者研究了花鲈人工培育及促熟催产中的技术措施。采用海捕的天然苗种，逐年筛选、精心培育，共获得4～6龄的成熟亲鱼50尾。在外源激素HCG（700～1 500 IU/kg）及LHRH-A3（2～5 μg/kg）的作用下（雄鱼减半），共人工采卵1 560×104粒，卵的平均上浮率为61%，平均受精率为78%，平均孵化率为85.7%。
关键词 花鲈；亲鱼培育；催产
中图法分类号 S961.3 文章编号 1001-1862(2001)02-195-06
Study on Artificial Cultivation and Spawning Inducement Technique of Lateolabrax japonicus
Zhang Meizhao1 Gao Tianxiang1 Ruan Shuhui2 Zhuang Qianzeng3 Qu Yongqi2
(1 College of Fisheries, Ocean University of Qingdao, Qingdao,266003)
(2 Aquaculture General Corporation of Yangting of Weihai,Weihai,264200)
(3 Shandong Institute of Mariculture Reseach,Qingdao,266002)
Abstract Artificial cultivation and spawning inducement technique of Lateolabrax japonicus were studied.50 matured parent fish of 4～6 years were obtained by choosing and cultivating natural seed carefully year after year. Under the action of outer hormone HCG(700～1 500 IU/kg) and LHRH-A3(2～5μg/kg)(male fish halved), we gained 1 560×104 eggs artificially, the average float-rate, fertilization-rate, and hatch-rate were 61%, 78%, and 85.7% respectively.
Key words Lateolabrax japonicus; parent fish cultivation; spawning inducement
PCR法制备地高辛标记探针斑点杂交检测白斑综合症病毒(WSSV)
国家“九五”科技攻关项目(96-005-03-01-02)资助
收稿日期：2000-07-06；修订日期：2000-11-02
雷质文，男，1970年8月出生，硕士。现在青岛出入境检验检疫局工作。
雷质文 史成银2 黄 倢 杨 冰 俞开康 战文斌
(1 青岛海洋大学水产学院，青岛，266003)
(2 中国水产科学研究院黄海水产研究所，青岛，266071)
摘 要 用PCR法成功制备了DIG标记探针，探针长度为547bp，探针的产量为21.6 ng/μL。此探针与随机引物合成探针检测样品灵敏度相近。用此探针核酸斑点杂交法检测了54尾中国对虾。结果表明：此探针在对白斑综合症病毒的检测、对虾暴发性流行病的诊断等方面具有很高的应用价值。
关键词 多聚酶链式反应(PCR)；核酸探针；白斑综合症病毒(WSSV)；中国对虾
中图法分类号 S945.1；S945.4 文章编号 1001-1862(2001)02-201-04
Development of Digoxigenin Labeled Probe
for Detection of White Spot Syndrome by Dot-blot HybridizationꗬÁ‹Љ勰¿ကЀ
橢橢쿽쿽ࠄ㐼ꖟꖟ阔lÀÀÀÀÀÀÀnj⡼⡼⡼⡼ ⢘„nj秒IJ⤨⤨⤨⤨⤨⨉⨉⨉瘭[1]瘯瘯瘯瘯瘯瘯$笄Ƞ紤X癓̹À⨉⨉⨉⨉⨉癓igenin labeled DNA
probe was produced by PCR. The size of probe was 547bp, and its production was
21.6ng/μL. The results of dot blot hybridization by the
probe developed by PCR were the
same as those by the probe of kit produced by random primer, their
sensitivity were similar. Using the probe produced by PCR to detection 54 P.
chinensis by dot blot hybridization showed that the probe produced by PCR was
h-a valuable in detecting WSSV and diagnosing explosive epidemic disease.
Key words PCR DNA probe; WSSV; Penaeas chineneis
十四株海洋微藻脂肪酸组成的研究
国家海洋863高新技术发展计划项目(863-819-02-01)资助
收稿日期：2000-01-20；修订日期：2000-10-25
张小葵，女，1966年11月生，硕士，讲师。
张小葵 麦康森 郭 健 孙世春
(青岛海洋大学教育部海水养殖重点实验室，青岛，266003)
摘 要 对4个门的14株(红藻门8株，甲藻门1株，隐藻门2株，绿藻门3株)海洋微藻进行了脂肪酸含量测定，微藻在确定的条件下生长，指数生长末期收获。结果表明，各门的微藻都有其独特的脂肪酸特征：红藻中含有大量的20:4n-6和20:5n-3，其中紫球藻R25含量最高，占总脂肪酸的49.8%(AA占总脂肪酸的20.5%,EPA占总脂肪酸的29.3%)。隐藻的主要脂肪酸是16:0、18:1n-9、18:3n-3、18:4n-3、20:5n-3、22:5n-3。与其它甲藻有别的虫黄藻，18:4n-3含量很少并且不含EPA，其主要合成的是16:0、18:1n-9和22:6n-3。C16和C18的不饱和脂肪酸是本实验研究的3株绿藻的主要脂肪酸。
关键词 微藻；脂肪酸；总脂
中图法分类号 Q547；Q949.2 文章编号 1001-1862(2001)02-205-06
Fatty Acid Composition of 14 Strains of Marine Microalgae
Zhang Xiaokui Mai Kangsen Guo Jian Sun Shichun
(The Key Laboratory of Mariculture, Ministry of Education, Ocean University of Qingdao, Qingdao, 266003)
Abstract The Fatty acid (FA) composition of 14 strains of marine microalgae from four taxonomic divisions were examined. The microalgae were grown under defined conditions and harvested during a late log phase. The result indicated that each phylum of microalgae was characterized by a specific fatty acid profile. Significant concentrations of 20:4n-6 and 20:5n-3 were present in the red microalgae, Porphyridium sp. with the highest proportion in R25 (49.8%). The major fatty acids in two species of Chroomonas were 16:0, 18:1 18:3n-3, 18:4n-3 and 20:5n-3. In Zooxanthella microadriatica, the fatty acid composition is different from that of other dinoflagellates. Z. microdriatica contains little 18:4n-3 and no EPA, its major fatty acids are 16:0, 18:1 and 22:6n-3. 16 and 18carbon unsaturated fatty acids are important components in 3 green alga strains examined.
Key words microalgae; fatty acid; total lipid
间接酶联免疫吸附技术检测活的非可培养状态的大肠杆菌O157：H7
联合国教科文组织项目(UNESC 861.375.9)资助
收稿日期：2000-04-25；修订日期：2000-09-30
姚 斐，女，1972年出生，硕士。
姚 斐 沙 莎 陈 刚 纪伟尚
（青岛海洋大学生命学院，青岛，266003）
（1 四川畜牧兽医学院微生物室，荣昌，402460）
摘 要 大肠杆菌O157：H7在低温贫营养的条件下可进入活的非可培养状态（Viable but nonculturable state,VBNC），用常规的培养法无法将其检出。作者利用间接酶联免疫吸附技术（Indirect Enzyme-Linked Immunosorbent Assay, ELISA）检测VBNC的大肠杆菌O157：H7，发现此方法可以快速有效地将其检出，最小检测浓度为105个/mL。
关键词 大肠杆菌O157：H7；活的非可培养状态；间接酶联免疫吸附技术
中图法分类号 Q93-335；Q932 文章编号 1001-1862(2001)02-211-04
Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Viable but Nonculturable Escherichia Coli O157:H7
Yao Fei Sha Sha1 Chen Gang Ji Weishang Xu Huaishu
(College of Marine Life Science, Ocean University of Qingdao, Qingdao, 266003)
(1 Department of Biology Technology,Veterinary Institute of Sichuan Province, Rong Chang, 402460)
Abstract Escherichia coli O157:H7 can enter the viable but nonculturable state (VBNC) under low temperatures and poor nutrition, the VBNC bacteria can not be detected by the traditional culturable methods. In this paper we use ELISA to detect the VBNC Escherichia coli O157:H7, the result shows that ELISA is a fast and efficient method to detect the VBNC bacteria, and the minimal concentration of bacteria that can be detected is 105cells/mL.
Key words Escherichia coli O157:H7; viable but nonculturable state; indirect enzymelinked immunosorbent assay
甘糖酯对大鼠尿激酶型纤溶酶原激活物活性的诱导作用
国家“九五”科技攻关项目(96-C02-02-01)资助
收稿日期：2000-02-28；修订日期：2000-04-18
姚子昂，男，1971年11月出生，博士生。
姚子昂 马洪明 姜招峰 管华诗
(青岛海洋大学 1海洋生命科学学院 2海洋药物与食品研究所, 青岛，266003)
摘 要 为了研究甘糖酯对大鼠体内尿激酶型纤溶酶原激活物(urokinase Plasminogen Activator，uPA)活性的影响，采用溶解圈中和抑制法和发色底物法检测了大鼠口服甘糖酯后体内uPA活性的变化情况。结果表明：甘糖酯可提高大鼠体内血液的纤溶活性，主要表现为uPA活性升高。以不同剂量的甘糖酯连续喂药10d后，发现喂药组大鼠uPA活性明显高于对照组，喂药组大鼠血浆uPA活性升高同喂药剂量正相关，在0～100 mg/kg的剂量范围内，随喂药剂量增加而升高。提示在一定的剂量范围内甘糖酯可提高大鼠体内uPA活性，激活纤溶系统，提高血液纤溶活性，表现出良好的抗栓作用。
关键词 甘糖酯； 尿激酶型纤溶酶原激活物； 大鼠； 活性
中图法分类号 R282.77 文章编号 1001-1862(2001)02-215-05
The Effect of Propylene Glycol Mannate Sulfate (PGMS)on the Activity of Rat uPA
Yao Zi'ang1 Ma Hongming2 Jiang Zhaofeng2 Guan Huashi2
(1 College of Marine Life Science; 2 Institute of Marine Drugs and Foods, Ocean University of Qingdao, Qingdao, 266003)
Abstract To study the effect of Propylene Glyco Mannate Sulfate (PGMS) on the activity of uPA, we detected the activity of uPA in rat blood before and after giving PGMS to rat. The result showed that PGMS could raise the fibrinolysis activity in rat blood, which was resulted from the rise of uPA's activity; After giving different doses of PGMS to rat for 10 days, the uPA activity of experiment groups was higher than that of the control group and increased significantly in a dose-dependant manner within the dose of 100 mg/kg, versus control group P＜0.05. So we draw the conclusion that PGMS can induce the increase of uPA activity in rat blood and activate the fibrinolysis system.
Key words Propylene Glycol MannateꗬÁ‹Љ勰¿ကЀ
橢橢쿽쿽ࠄ㐼ꖟꖟ阔lÀÀÀÀÀÀÀnj⡼⡼⡼⡼ ⢘„nj秒IJ⤨⤨⤨⤨⤨⨉⨉⨉瘭[1]瘯瘯瘯瘯瘯瘯$笄Ƞ紤X癓̹À⨉⨉⨉⨉⨉癓数的模板，对其进行白斑症病毒(WSSV)的PCR扩增，结果显示所建立的套式PCR的灵敏度大约为一步PCR的104倍；对感染病毒后不同时期的斑节对虾进行PCR检测，发现感染早期经一步PCR检测为WSSV阴性的样品，套式PCR的检测结果为阳性；对发病虾塘中的几种甲壳类动物进行PCR检测，发现经一步PCR检测为阴性的长臂虾、秉氏厚蟹和褶痕相手蟹等宿主，套式PCR的检测结果为阳性。表明所建立的WSSV套式PCR检测法较普通的一步PCR有更大的应用价值和意义。
关键词 白斑症；白斑症病毒；斑节对虾；套式PCR
中图法分类号 S945.4+7； S945.4 文章编号 1001-1862(2001)02-220-05
Detection of White Spot Syndrome Virus(WSSV) in Penaeus Monodon Using Nested PCR
Xie Shutao He Jianguo Yang Xiaoming Lu Ling Jiang Jingbo
(1 School of Life Sciences, Zhongshan University, Guangzhou 510275;2 Deptment of Veterinary Medicine,
South China Agricultural University, Guangzhou 510642)
Abstract Based upon the sequence of WSSV DNA fragment, two specific primer sets for nested PCR were designed. We evaluated the effect of the nested PCR with detecting WSSV using templates with different dilutions from diseased shrimp gill. The sensitivity of the nested PCR was about 104 times higher than that of one step PCR. The results of screening early infected shrimps or WSSV hosts show that we provide a more effective diagnostic tool in detecting WSSV.
Key words white spot syndrome; white spot syndrome virus(WSSV); Penaeus monodon; nested PCR
弧菌拮抗菌的筛选
国家“九五”科技攻关项目（96-005-03-01-03）资助
收稿日期：1999-12-25；修订日期：2000-07-28
莫照兰，女，1967年6月出生，博士后。
莫照兰 俞 勇 李会荣 李 筠 纪伟尚 徐怀恕
徐怀恕为本文责任作者。
（青岛海洋大学海洋生命学院，青岛，266003）
摘 要 用平板划线或点种法对602株海洋细菌进行筛选，得到4株对海水养殖动物(鱼、虾、贝)的病原菌有拮抗作用的细菌。并对拮抗菌QJ2的拮抗作用进行了研究，结果为：QJ2有广泛的弧菌抗菌谱，对37株弧菌的抗菌阳性率达到89.2%(33/37)；用活菌平板计数法和O.D.600nm研究了QJ2培养物的去细胞上清液（CFS）与病原菌W-1的作用动力学，15min时W-1的细菌数便开始减少，4h时细菌数最少，6h后开始增加，而对照组的细菌数呈逐渐上升趋势；QJ2对自身的拮抗物质不敏感；QJ2抗性物质的分子量不大于8000Da；经常规生理生化方法和API-20细菌快速鉴定系统鉴定，QJ2为气味黄杆菌(Flavobacterium odoratum)。
关键词 拮抗菌；病原性弧菌；抗菌谱；气味黄杆菌
中图法分类号 Q938.1 文章编号 1001-1862(2001)02-225-07
Selection of VibriosAntagnism Bacteria
Mo Zhaolan Yu Yong Li Huirong Li Yun Ji Weishang Xu Huaishu
(College of Marine life Sciences, Ocean University of Qingdao,Qingdao, 266003)
Abstract Four antagonistic bacteria were selected from 602 strains by their inhibitory activities to 4 indicating vibrios. Evident clear zones or overlaying zones were observed around the selected bacteria by dot or scratch-inoculating method. Strain QJ2 had a wide antibacterial spectrum and was able to inhibit 33 vibrios among 37. Study of kinetic antibacterial activities of QJ2 against pathogen W-1 was tested by viable bacteria counts and absorbency at 600nm: 106～ 107 cfu/mL cells of W-1 began to decrease in 15 min after mixing with CFS(cell free-supernatant) of QJ2; the bacterial counts decreased to a minimum at 4h, and the numbers of bacteria began to increase after 6h. The cells of QJ2 did not show any sensitivity to its inhibitory substances in CFS. The size of active molecule in the CFS of QJ2 was smaller than 8000 Da by measuring the inhibitory activities of the fluid inside and outside the dialysis cellulose tubing. QJ2 was identified as Flavobacterium ordaratum by traditional morphological and biochemical method and API-20 identification system. Key words antaganistic bacteria; vibrio pathogens; antibacterial spectrum; Flavobacterium odoratum
增加贝类染色体分裂相的方法初探
国家海洋“863”计划课题(819-04-07)资助，国家重点基础研究发展规划项目课题(G1999012009)部分资助
收稿日期：1999-09-20;修订日期：2000-05-31
贾志良，男，1972年12月出生，硕士研究生。
贾志良 李智盈 包振民通讯联系人 潘 洁 王如才
（青岛海洋大学，青岛，266003）
摘 要 研究以太平洋牡蛎为材料，用低温同步化法和8 μg/mL的植物血球凝集素(PHA-M)、50 μg/mL伴刀豆蛋白A(ConA)两种外源凝集素分别处理其鳃，后用鳃做染色体滴片，10%Giemsa染色后镜检，统计分裂相数。发现三种方法都能使分裂相比例增加，其中低温同步化法效果最好，可使分裂相比例由对照的0.7×10升高到6.5×10，提高8.3倍。PHA-M法使分裂相比例由对照的0.7×10升高到5.5×10, 提高6.8倍，ConA法使分裂相比例由对照的0.7×10升高到3.6×10，提高4.1倍。
关键词 太平洋牡蛎；分裂相比例；低温同步化法；外源凝集素
中图法分类号 S944.4+1；Q343.2+4 文章编号 1001-1862(2001)02-232-05
On Methods of Increasing the Metaphase of Shellfish
Jia Zhiliang Li Zhiying Bao Zhenmin Pan Jie Wang Rucai
(Ocean University of Qingdao, Qindao,266003)
Abstract Three methods, low temperature synchronizing and two lectins—8μg/mL PHAM and 50μg/mL ConA, were used to treat the gill of Pacific oyster (Crassostrea gigas). The gill was dropped on a slide, stained with 10% Giemsa and then examined with microscope for the metaphase to be counted. The proportion of metaphase increased after the gill was treated by all the three methods. We find that low temperature synchronizing is the best one. It can increase the proportion from 0.7×10 to 6.5×10, which means an increase of 8.3 times. PHA-M treatment can increase the proportion from 0.7×10 to 5.5×10, the increase is 7.8 times, and ConA treatment can increase the proportion from 0.7×10 to 3.6×10, which is 4.1 times.
Key words Pacific oyster;proportion of metaphase;low temperature synchronizing;lectin
黄河三角洲河道沉积规律研究Ⅱ.建林边滩沉积作用机理
国家“九五”科技攻关课题“典型河口冲淤灾害预测技术研究”（96-922-03-02）资助
收稿日期：1999-10-28；修订日期：2000-06-05
范德江，男，1965年3月出生，副教授。
范德江 陈彰榕 栾光忠
（青岛海洋大学海洋地球科学学院，青岛，266003）
摘 要 通过对建林边滩沉积物粒度分布、粒度参数及水体流变性质的分析，阐述了黄河三角洲上河道水流属于牵引流范畴，与低含沙水流无本质上的差别，并探讨沉积物搬运和沉积的基本特征。
关键词 黄河三角洲; 河道; 粒度分布; 沉积机理
中图法分类号 P56 文章编号 1001-1862(2001)02-237-06
Riverway Sedimentation over the Yellow River Delta Ⅱ.The Sedimentation Mechanism of the Jianlin Sediment Bar
Fan Dejiang Chen Zhangrong Luan Guanzhong
(College of Marine Geosciences, Ocean University of Qingdao, Qingdao, 266003)
Abstract The Jianlin Sedimentary Bar, which is our study area, is
located in the modern Yellow River Delta. Nearly 50 sedimentary samples were
collected from the bar and the river bed. The components of grain size of the
samples were analyzed with sieving and Stoke's method. The grain size
distribution and their relative parameters suggested that the movement of the
Yellow River water is the same as the movement of water with low content
suspended maꗬÁ‹Љ勰¿ကЀ
橢橢쿽쿽ࠄ㐼ꖟꖟ阔lÀÀÀÀÀÀÀnj⡼⡼⡼⡼ ⢘„nj秒IJ⤨⤨⤨⤨⤨⨉⨉⨉瘭[1]瘯瘯瘯瘯瘯瘯$笄Ƞ紤X癓̹À⨉⨉⨉⨉⨉癓凸起的含油气性
收稿日期：1999-08-10；修订日期：2000-07-27
肖国林，男，1962年4月出生，硕士，副研究员。
肖国林
(国土资源部海洋地质研究所，青岛，266071)
摘 要 勘探资料证实，西湖凹陷内生成的油气由生烃深凹向西部和南部的古斜坡带运移。凹陷内的局部构造大多形成于中新世末期的龙井运动Ⅱ幕，圈闭形成期晚于油气主运移期，致使油气运移到比斜坡带更高的海礁凸起上保存或散失了。作者利用现有东海地震资料及西湖凹陷钻井、测井及油气资源评价结果从油气运移、聚集、保存等方面探讨东海陆架盆地海礁凸起油气勘探的良好前景。
关键词 油气勘探；海礁凸起；东海陆架盆地
中图法分类号 P736.5 文章编号 1001-1862(2001)02-243-06
Discussion on the Hydrocarbon Potential in Haijiao Uplift of the East China Sea Basin
Xiao Guolin
(Institute of Marine Geology, MLNR, Qingdao, 266071)
Abstract The exploration data approve that the hydrocarbon in the Xihu Depression migrated from the deep depression of hydrocarbon--generating to the west and south parts of the ancient slope. The most of the local structures discovered in the depression were formed in the 2nd Episode of the Longjing Movement, and the formed traps stage is later than the oil-gas migration peak-stage, causing oil-gas to migrate into the Haijiao Uplift, which is higher than the slope zone, and it was preserved or escaped. The good prospects of hydrocarbon exploration in the East China Sea Shelf Basin are discussed from the migration accumulation and preservation etc of hydrocarbon in the Haijiao uplift in this paper.
Key words hydrocarbon exploration; Haijiao uplift; East China Sea Shelf Basin
现代长江、黄河河口水下三角洲不稳定性的对比研究
收稿日期：1999-10-16 修订日期：2000-07-03
陈卫民，男，1963年2月出生，副教授。
陈卫民 杨作升
(青岛海洋大学, 青岛, 266003)
摘 要 现代长江口、黄河口水下三角洲地区的海底上发育有大量规模形态各异的不稳定性现象。其中沙波和浅埋的生物气体是长江口水下三角洲发育的主要形态，而滑坡和一些冲蚀结构则是黄河口水下三角洲的主要类型；它们在分布上也有较大的差异，长江水下三角洲主要呈垂直岸线分布，而在黄河水下三角洲则表现为平行岸线分布；分析认为这些现象与其河口的沉积环境和动力机制密切相关。
关键词 长江； 黄河； 不稳定性； 水下三角洲
中图法分类号 P56 文章编号 1001-1862(2001)02-249-07
Contrasting Study of the Subaqueous DeltaInstability of the Changjiang and Huanghe River
Chen Weimin Yang Zuoshen
(Ocean University of Qingdao, Qindao, 266003)
Abstract There are a great many different scale and shape unstable features developed on the subaqueous delta of the modern Changjiang River and Huanghe River. Sandwave and shallow buried gas are the main types on the Changjiang River subaqueous delta, but on the Huanghe River subaqueous delta, they are landslide and erosioned textured bottom mainly. And they have great difference in the distribution too: they are distributed on the Changjiang River subaqueous delta perpendicular to the coast, but on the Huanghe River subaqueous delta they are parallel to the coast. This is propably connected with esturine environment and dynamic machanisms.
Key words Changjiang (Yang·tze) River; Huanghe (Yellow) River; instability; subaqueous delta
过硫酸盐氧化法测定海水中溶解总磷
国家自然科学基金重大项目（497901001）和山东省自然科学基金课题（Q95B0314）资助
收稿日期：1999-10-08；修改日期：2000-05-08
谭加强，男，1976年9月出生，硕士。
谭加强 于志刚 林桂炽 米铁柱
（青岛海洋大学化学化工学院，青岛，266003）
摘 要 实验研究在碱性和酸性条件下过二硫酸钾高温氧化四种不同类型含磷标准化合物在海水中的回收率和影响回收率的因素。结果表明：在碱性条件下过二硫酸钾高温氧化可使海水中非聚磷类化合物完全回收，而聚磷类化合物回收率不高，延长消化时间聚磷类化合物的回收率有一定提高。除个别化合物外，各类含磷化合物在酸性条件下可被定量回收。依此，建立酸性过硫酸盐氧化法分解测定海水中溶解态总磷的方法。
关键词 溶解态总磷；过硫酸盐氧化；回收率；海水
中图法分类号 P734 文章编号 1001-1862(2001)02-256-07
The Decomposition and Analysis Method for Dissolved Total Phosphorus in Sea Water by Persulphate Oxidation
Tan Jiaqiang Yu Zhigang Lin Guichi Mi Tiezhu
(College of Chemistry and Chemical Engineering，Ocean University of Qingdao，Qingdao，266003)
Abstract Experimental studies have been conducted on the decomposition efficiency of four different types of organic and inorganic phosphorus compounds in seawater under acid or basic condition by persulphate oxidation. The influence factors on decomposition efficiency, such as digestion time and pH were identified. It was shown that non-condensed phosphate could be decomposed completely by persulphate. But in alkali condition, condensed phosphate can only be decomposed partly. Almost 100% decomposition efficiency could be reached for all types of compounds containing phosphorus under acid condition. A persulphate oxidation method in acid medium for decomposition and analysis of dissolved total phosphorus in seawater was proposed.
Key words dissolved total phosphorus; persulphate oxidation; recovery; seawater
羧甲基壳聚糖与表面活性剂的相互作用
山东大学‘胶体与界面化学’教育部开放实验室课题资助
收稿日期：2000-02-29；修订日期：2000-11-15
隋卫平，女，1966年7月出生，博士生，讲师。现在济南大学化学系任教。
隋卫平 陈国华 杨 爽 王宗春
（青岛海洋大学化学化工学院，青岛，266003）
摘 要 作者从配伍稳定性、表面张力、粘度等方面研究羧甲基壳聚糖（CMCHS）与四种典型类型表面活性剂的相互作用情况。研究显示，羧甲基壳聚糖与阴离子表面活性剂SDS、非离子表面活性剂TritonX-100、两性表面活性剂C12BE配伍稳定性较好，而与阳离子表面活性剂CTAB及C16PyBr复配时则在一定的浓度区会产生沉淀。羧甲基壳聚糖可使SDS表面活性升高，而SDS则使羧甲基壳聚糖比浓粘度下降；CTAB可与羧甲基壳聚糖形成复合物，从而使表面张力曲线产生两个转折点，即使在沉淀区仍有较好的表面活性，而溶液的比浓粘度则大幅度下降；TritonX-100 和C12BE与羧甲基壳聚糖的相互作用不很明显。
关键词 羧甲基壳聚糖（CMCHS）；表面活性剂；相互作用；配伍稳定性
中图法分类号 P734；Q647.2；O636.1 文章编号 1001-1862(2001)02-263-06
Interaction Between Carboxymethylchitosan and Various Surfactants
Sui Weiping Chen Guohua Yang Shuang Wang Zongchun
(College of Chemistry and Chemical Engineering,Ocean University of Qingdao, Qingdao, 266003)
Abstract The interaction between carboxymethylchitosan and various kinds of surfactants have been studied by means of compatibility, surface tension and relative viscosity. It was found that there was good compatibility between CMCHS and SDS, TritonX-100 and C12BE, but the solution of CMCHS and CTAB could cause precipitation in some range of concentration. CMCHS could increase the surface activity of SDS, while SDS could decrease the viscosity of CMCHS. The formation of complex between CMCHS and CTAB caused the surface tension curve to form two-transitions and the viscosity decreased dramatically. There was no obvious interaction between CMCHS and TrotonX-100 and C12BE.
Key words Carboxymethylchitosan(CMCHS); surfactants; interaction; compatibility
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关键词 胶体粒子；金属；含量；大亚湾
中图法分类号 P734.3+1 文章编号 1001-1862(2001)02-269-06
Significance of Colloids in the Transport and Transformation of Trace Metals at Daya Bay
Li Li Zhang Zhengbin Wang Zhaoding
(Institute of Marine Chemistry, Ocean University of Qingdao, Qingdao,266003)
(1 Marine Biology Research Station at Daya Bay,South China Sea Institute of Oceanology, Academia Sinica, Guangzhou,510301)
Abstract Cross-flow ultrafiltration techniques have been used to extract colloids(0.45μm~103Daltons) at Daya Bay and the recoveries were calculated. We determined the concentration of Cu,Pb and Cd in colloidal and dissolved (＜103Daltons) trace metals. On average, approximately 14%Cu,20%Pb and 24%Cd were actually associated with colloid,which confirmed that the significant role of colloidal materials in the transport and transformation of trace metals.
亚硝酸测定工艺流程优化及试剂稳定性研究
国家海洋“863”项目(818-01-01-03)资助
收稿日期：1999-10-11；修订日期：2000-09-25
秦延文，女，1973年9月出生，硕士生。
秦延文 张曼平 欧明明
（青岛海洋大学化学化工学院，青岛，266003）
李世珍 邹常胜
（国家海洋局海洋技术研究所，天津，300111）
摘 要 对《海洋调查规范》中测定海水NO2-N的常规方法进行了优化，达到简化操作过程，缩短分析时间以适应水下现场自动分析的目标。此外还对亚硝酸盐测定所使用的试剂及标准贮备液的稳定性进行了一系列的研究，改进试剂保存方法，延长试剂使用寿命，以满足长期现场测定的要求。
关键词 亚硝酸盐；测定工艺优化；试剂稳定性；海洋调查规范
中图法分类号 P714+.4 文章编号 1001-1862(2001)02-275-06
Optimization of Chemical Determination Process of Nitrite and Study on the Reagents Stability
Qin Yanwen Zhang Manping Ou Mingming
(College of Chemistry and Chemical Engineering, Ocean University of Qingdao, Qingdao, 266003)
Li Shizhen Zou Changsheng
(Institute of Ocean Technology, State Oceanic Administration, Tianjin,30011)
Abstract The routine method of nitrite determination in seawater in “The Marine Survey Norm” has been optimized in this paper, which will simplify the operation processes and shorten the analytical time in order to adapt to the demands of auto-determination under water. Besides, the stability of reagents and nutrient standard stock solution has been studied in the determination of nitrite in seawater in situ for improving the preservation methods and prolonging the service life of reagents to meet the long-time demands of auto-analyzer.
Key words Nitrite; optimization of determination-process; stability of reagents; specifications of oceanographic survey
具有小时滞的线性系统次优控制的无滞后转换法
国家自然科学基金课题(60074001)资助
收稿日期：2000-09-16；修订日期：2000-11-16
王 芳，女，1974年2月出生，硕士生。
王 芳 唐功友
(青岛海洋大学计算机科学系，青岛，266003)
摘 要 该文研究线性时滞定常系统的次优控制问题。根据无滞后转换法的思想，先引入状态向量的增量，将其视为附加扰动输入，再利用微分方程的逐次逼近法，将既含有时滞项又含有超前项的两点边值问题化为既不含时滞项又不含超前项的两点边值问题族。然后，把第N次逼近得到的控制律近似为系统的最优控制律，得到次优控制律。并用实例仿真验证了该算法的有效性。该方法可使小时滞系统的迭代次数大大减少，因此尤其适合于小时滞系统的次优控制。
关键词 线性时滞系统；最优控制；次优控制；无滞后转换法
中图法分类号 O231.1 文章编号 1001-1862(2001)02-281-06
Non-Delay Transformation Approach of Suboptimal Control for Linear Systems with Small Time-Delay
Wang Fang Tang Gongyou
(Department of Computer Science, Ocean University of Qingdao, Qingdao, 266003)
Abstract In this paper the suboptimal control of linear systems with time-delay is studied. We introduce an increment of state terms as an additional disturbance, and use the asymptotic approximation method in differential equation theory to solve the problem. We transform the two-point boundary value problem with time-delay terms and time--advance terms to a group of problems without time-delay terms and time-advance terms according to the concept of non-delay transformation approach. By taking the N iterative value of the optimal solutions, we obtain a suboptimal control law. A simulation example is given to test the validity of the algorithm. When the system of the time-delay is small, the number of iterative times may be decreased greatly. Therefore the method is more suitable for the suboptimal control of small time-delay systems.
Key words linear timedelay systems; optimal control; suboptimal control; non-delay transformation approach
Oscillation of Certain Difference Equations
This work was supported by NNSF of China
Received: 2000-07-03; Revised: 2000-12-04
Sun Yijun(孙亦君) Zhang Binggen(张炳根)
(Department of Mathematics, Ocean University of Qingdao, Qingdao, 266003)
Abstract In this paper, we investigate a class of higher order difference equations and present some oscillation criteria.
Key words difference equations; oscillation; positive solutionsAMS Subject Classification 39A
差分方程的振动性
国家自然科学基金课题(1977105-1)资助
收稿日期：2000-07-03; 修订日期：2000-1204
孙亦君，女，1976年2月出生，硕士生。
孙亦君 张炳根
(青岛海洋大学数学系，青岛，266003)
摘 要 作者研究差分方程Δx(t)+p(t)Δx(t)+H(t,x(g(t)))=0,其中 P∶D→R, H∶D×R→R，0≤p(t)≤1, g∶D→D, limt→∞t∈D g(t)=∞, D={t0, t0+1, t0+2,…}.得到保证这个方程的一切解都振动的若干充分条件。
关键词 差分方程；振动性；正解
中图法分类号 O241.3 文章编号 1001-1862(2001)02-287-06
多圆环柱域上解析函数边值问题再论
收稿日期：1999-11-17；修订日期：2000-12-08
王莉萍，女，1963年7月出生，硕士，副教授。
王莉萍
(青岛海洋大学数学系，青岛266003)
摘 要 讨论多圆环柱域上多个复变量的解析函数的一般形式的Riemann-Hilbert边值问题。通过函数的解析变换，导出了解析函数一般形式的Riemann-Hilbert边值问题的可解性，并给出了解的积分表达式。
关键词 多圆环柱域；解析函数；Riemann-Hilbert边值问题
中图法分类号 O174.56 文章编号 1001-1862(2001)02-293-04
Further Discussion on the Boundary Value Problem
for Analytic Functions in Several Circular Ring Cylinder Domain
Wang Liping
(Mathematics Department, Ocean University of Qingdao, Qingdao, 266003)
Abstract In this paper,we discuss the general case of the
Riemann-Hilbert boundary value problem for analytic functions of several
complex variables in several circular ring cylinder domain.Through the
transformation of analytic functions, we have derived the solvability for the
ordinary Riemann-Hilbert boundary value problems for an analytic function of
several complex variables. And wꗬÁ‹Љ勰¿ကЀ
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